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For E2F1 and histone ChIP-seq, the chromatin was immunoprecipitated with appropriate amounts of antibody. Extracted molecule genomic DNA Extraction protocol Cells were cross-linked then sonicated to generate chromatin fragment sizes between 250-500bp.

UC-MSCs were cultured in Dulbecco’s modified Eagle’s medium containing high glucose which was further supplemented with 20% fetal bovine serum, 1 X Insulin-Transferrin-Selenium G, 5 ng/ml basic fibroblast growth factor, 1 X Antibiotic/antimycotic mix and 0.1 mmol/l beta-mercaptoethanol. Scope: Format: Amount: GEO accession: Status Public on Title ID44.H3K4me1 Sample type SRA Source name umbilical cord wharton's jelly Organism Characteristics chip antibody: H3K4me1 (2ug Abcam, AB8895) number of cells: 1.5 million cell type: Mesenchymal stem cells agasga: AGA Growth protocol UC-MSCs were cultured in Dulbecco’s modified Eagle’s medium containing high glucose which was further supplemented with 20% fetal bovine serum, 1 X Insulin-Transferrin-Selenium G, 5 ng/ml basic fibroblast growth factor, 1 X Antibiotic/antimycotic mix and 0.1 mmol/l beta-mercaptoethanol.

Just drag the file onto this browser window and drop it.GEO help: Mouse over screen elements for information. You can also display a DOW file directly in the browser. Then click "Open with" and choose an application. If you cannot open your DOW file correctly, try to right-click or long-press the file. Without proper software you will receive a Windows message " How do you want to open this file?" or " Windows cannot open this file" or a similar Mac/iPhone/Android alert. You need a suitable software like TAPCIS to open a DOW file. DOW file is created as software in libraries are marked for download in the.

The program was written and supported by Howard Benner and OMNI Information Resources, Inc. The program dates from the 1983 timeframe where it started as an interpreted BASIC program and later became a standalone program. TAPCIS is a DOS-based access program for the CompuServe Information Service.